A smartphone-read ultrasensitive and quantitative saliva test for COVID-19

Authored by advances.sciencemag.org and submitted by mvea

Abstract Improved point-of-care COVID-19 assays are urgently needed to improve disease control efforts, but current assays lack the sensitivity of the RT-PCR gold-standard. Herein we describe the development of a portable, ultrasensitive saliva-based COVID-19 assay with a 15 minute sample-to-answer time that does not require RNA isolation or laboratory equipment. This assay uses CRISPR/Cas12a activity to enhance signal from an amplified viral RNA target, which is stimulated by laser diode integrated into a smartphone-based fluorescence microscope readout device. This device robustly quantified viral load over a broad linear range (1-105 copies/μL) and exhibited a limit of detection (0.38 copies/μL) below that of the RT-PCR reference assay. SARS-CoV-2 RNA levels read by CRISPR were similar in patient saliva versus nasal swab samples and viral loads measured by RT-PCR and the smartphone-read CRISPR assay demonstrated good correlation, supporting the potential utility of this portable assay device for saliva-based point-of-care COVID-19 diagnosis.

Introduction SARS-CoV-2 has rapidly spread from its initial outbreak site to produce a pandemic (1) that has caused >674,000 deaths within its first 7 months (2), but accurate, sensitive and large-scale testing for SARS-CoV-2 still presents a challenge for ongoing disease control efforts. New assays required to expand COVID-19 testing capacity should 1) use samples that can be easily collected 2) have greater sensitivity than the current reference standard, and allow viral quantification for treatment monitoring, and 3) require minimal training and equipment to obtain valid, robust and quantitative results from samples containing a broad range of virus concentrations. Most COVID-19 assays require nasopharyngeal swab samples that must be collected by in airborne infection isolation rooms by medical professionals wearing full protective gear. Nasal swab sample collection procedures may be better tolerated than those for nasopharyngeal swab samples, but it is not clear if nasal swab collection involves less transmission risk. Nor is it clear if these samples are comparable to nasopharyngeal swabs, as small studies have reported lower detection rates for respiratory viruses analyzed by Nucleic Acid Amplification (NAA) of nasal versus nasopharyngeal swab samples (3, 4). However, recent studies indicate that saliva and nasopharyngeal SARS-CoV-2 results exhibit correlation during early infection, and development of saliva-based COVID-19 assays could reduce or eliminate the involvement of medical personnel in sample collection, since saliva collection would not require special materials, training or infrastructure. Saliva samples offer practical and logistical advantages for diagnostic and screening efforts, since they can be directly collected by the patient, reducing the need for, and exposure risk of, medical personnel. Saliva samples also do not need to be collected in airborne infection isolation rooms, which allows collections in outpatient clinics, community testing sites, or other locations as needed to meet local needs. Both these features could improve potential testing bottlenecks and enhance diagnostic and screening efforts by reducing the labor required from medical professionals. Quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) is the primary means employed to diagnose COVID-19 using respiratory samples, and saliva (5, 6). However, RT-qPCR has several drawbacks for efforts to expand screening capacity: it requires technical expertise and expensive equipment, exhibits a significant false negative rate, and requires that trained personnel wear extensive personal protective equipment to safely collect valid diagnostic samples. New rapid nucleic acid tests, such as the Abbott ID Now system, do not require the technical expertise or sophisticated equipment necessary for RT-qPCR, and have the potential to expand COVID-19 diagnosis capacity outside traditional clinical laboratory settings. Unfortunately, the reported positive percent agreement rate of the Abbott ID Now system with RT-qPCR (73.9% ~ 80.4%) is unacceptable, due to its alarming false negative rate (7–9). New NAA strategies may, however, reduce the infrastructure and expertise required to obtain ultrasensitive diagnostic results. Isothermal NAA approaches, such as recombinase polymerase amplification (RPA) or loop-mediated isothermal amplification (LAMP), can reduce equipment demands, while CRISPR-Cas activity can be employed to enhance detection sensitivity (10–18). Several groups have now reported COVID-19 diagnostic assays that employ RT-RPA or RT-LAMP, with or without CRISPR-mediated target detection (14, 19–23), including studies that employed colorimetric lateral flow assay formats suitable for use in resource limited settings (14, 23). However, sensitivity limits reported for these assays (5~10 copies/μL) are higher than the reported limit of detection (LOD) for the RT-qPCR reference standard (1 copy/μL). Here we report the development of a RT-RPA CRISPR-Fluorescent Detection System (FDS) assay to sensitively quantify SARS-CoV-2 present in saliva, without RNA isolation, and adapted this assay to a chip format that was a smartphone reader. To accomplish this, we first optimized lysis buffer conditions required to reproducibly maximize signal from the subsequent CRISPR-FDS assay. Next, we determined the linear range and limit of detection or the optimized assay, evaluated the correspondence of CRISPR-FDS and RT-qPCR assay results upon analysis of healthy saliva samples spiked with and without SARS-CoV-2 RNA, and analyzed the correlation between viral RNA load in paired saliva and nasal swab samples obtained from patients with diagnosed with COVID-19. These studies were followed by CRISPR-FDS evaluation of the time course of SARS-CoV-2 RNA expression in nasal and pharyngeal swab samples of a non-human primate of COVID-19 and a comparison of CRISPR-FDS-quantified viral RNA load it correlation between paired nasal swab and saliva samples obtained from individuals screened for COVID-19. Finally, we adapted this CRISPR-FDS laboratory test to a chip-format assay read by a prototype smartphone-based fluorescent microscope device designed for point-of-care use, and evaluated its analytical performance and the agreement between smartphone-read CRISPR-FDS assay and RT-qPCR results for saliva samples obtained from patients screened for COVID-19. This saliva assay exhibited complete concordance with RT-qPCR results from paired nasopharyngeal samples, a low limit of detection, and a broad linear range, and thus appear suitable for ultrasensitive point-of-care COVID-19 screening efforts.

Results Optimization of CRISPR-FDS for extraction-free detection of SARS-COV-2 RNA in saliva A saliva-based point-of-care COVID-19 assay should, ideally, function as a single step assay that can be read without any additional equipment or infrastructure, while retaining high sensitivity (Fig. S1). Since CRISPR-FDS has high sensitivity using a simple workflow that requires minimal equipment, we used its assay protocol as a starting approach, and optimized aspects of its lysis procedure (sample-to-buffer ratio, and incubation temperature and time) and RT-PCR CRISPR reaction (Cas12/guide RNA(gRNA)-to-probe ratio, and reaction temperature and time) to improve workflow and performance (Fig. 1A). Fig. 1 Optimization of an RNA extraction-free assay for saliva-based COVID-19 diagnosis. (A) Optimization workflow for RNA recovery and CRISPR-FDS reaction efficiency. Steps 1-3: Sample lysis conditions were evaluated by CRISPR-FDS signal produced by healthy donor saliva (10 μL) spiked with heat-inactivated SARS-CoV-2 virus (100 copies/μL) after optimizing: 1) sample dilution with increasing lysis buffer (B: 1:1–1:10 saliva-to-buffer; 65°C for 5 min); 2) lysis temperature conditions (C: 22–95°C; 1:1 dilution for 5 min); and incubation times (D: 5, 10 or 15 min; 1:1 dilution at 65°C). NC: negative control (50 μL 100 ng/mL human RNA). Steps 4-6: CRISPR reaction efficiency was optimized by 4) titrating the Cas12a/gRNA-to-probe molar ratio (1:15–1:25 Cas12a/gRNA-to-probe (E: 42°C for 30 min); 5) altering reaction temperature (F: 22°C – 42°C; 30 min reaction with a 1:25 Cas12a/gRNA-to-probe ratio); and 6) evaluating the differ in signal from of a representative COVID-19 patient and healthy donor over time (G: 30 min at 42°C with a 1:25 Cas12a/gRNA-to-probe ratio) Data represent the mean ± SD of three technical replicates; ****, P<0.0001. Most high sensitivity NAA assays analyze purified RNA samples isolated in multi-step procedures that require additional laboratory equipment. This additional step must be eliminated to allow the development of an integrated assay. We thus optimized the viral lysis procedure to determine the conditions that would allow viral lysis samples to be directly analyzed by CRISPR-FDS without a separate isolation step, using a cell lysis procedure compatible with PCR as the base condition. CRISPR-FDS exhibited robust performance when healthy donor saliva samples spiked with SARS-CoV-2 were mixed with increasing lysis buffer volumes (1:1 to 1:10, saliva-to-buffer) prior to standard heat denaturation (65°C for 5 min) and direct analysis by CRISPR-FDS (Fig. 1B). SARS-CoV-2 RNA signal increased with lysis buffer concentration up to a 1:5 ratio. However, signal detected at the 1:1 and 1:10 ratios were similar, and ~40% less than the signal detected at the 1:5 ratio, indicating that CRISPR-FDS results were not markedly affected by the sample-to-buffer ratio. Given this robust response, we elected to use a 1:1 sample-to-buffer ratio for all further studies. Since temperature control can be a significant factor in point of care assays, we next analyzed the effect of lysis temperature on CRISPR-FDS signal. No signal was detected when lysis reactions were performed at room temperature (~22°C), similar CRISPR-FDS signal was detected at 37°C and at 65°C, the manufacturer recommended lysis temperature, with the most signal detected at 95°C (Fig. 1C). We next examined the effect of lysis time on CRISPR-FDS signal when using a 1:1 saliva-to-buffer ratio at 65°C and found that signal increased with incubation time out to the final 15 min time point (Fig. 1D). Given that there was no difference between 65°C and 37°C lysis, we elected to use 37°C for the lysis temperature, with the assumption that this minimum temperature would be the simplest to achieve, maintain and measure in most point-of-care settings, although temperatures deviations above this minimum would not negatively influence the lysis reaction. We also chose to use a 5 min lysis time to minimize overall assay performance time. We next evaluated CRISPR-FDS saliva assay performance at 42°C, the maximum recommended temperature for Cas12a reactions, varying reaction time and Cas12a/gRNA-to-probe ratio to determine the optimal conditions. Increasing probe concentration enhanced CRISPR-FDS signal at 37°C but increased reaction completion time, with 1:20 and 1:25 ratios requiring 20% and 40% longer, respectively, than 1:15 assays, while producing 35% and 70% more signal (Fig. 1E). Reducing the CRISPR-FDS reaction temperature increased assay completion times, but did not alter the final signal, with CRISPR-FDS assays performed at 42°C, 37°C, and 22°C reaching completion after 11, 15 and 27 min incubation, respectively (Fig. 1F). CRISPR-FDS signals detected after 10 min at 42°C, 37°C, and 22°C reached 97%, 84%, and 50% of the signal detected at reaction completion, respectively, allowing robust detection over a broad temperature range at this time point. We next evaluated the specificity of our RT-RPA-CRISPR assay using 39 negative control samples that each contained >106 genome copies/ml of different bacterial/viral/fungal species that can cause respiratory infections, including influenza A and B and respiratory syncytial virus (RSV). No positive results were detected for any of these negative control samples in triplicate assays (Table S3). Individual CRISPR-FDS assays could be generated for each of these respiratory infections by replacing the gRNA used in the current assay with gRNAs that recognize gene targets specific for each of these pathogens. Multiplex detection within a signal assay reaction is not feasible, however, since CRISPR-FDS utilizes the non-specific single-strand DNA cleavage activity of the bound Cas12a/gRNA-DNA complex to derepress the fluorescent signal from a quenched oligonucleotide probe. Based on these results we next examined the diagnostic potential of a rapid saliva-based CRISPR-FDS assay in which lysis (5 min; 1:1 sample to buffer) and CRISPR (10 min) incubation steps were performed at 37°C, using a 1:25 CRISPR/gRNA to probe ratio. CRISPR-FDS assay results obtained upon saliva from a patient with confirmed COVID-19 and from a healthy individual indicated that this assay can readily distinguish between these individuals (Fig. 1G). Evaluation of a saliva-based CRISPR-FDS assay for quantitative COVID-19 diagnosis Next, we analyzed the linear range and other properties of the optimized saliva-based CRISPR-FDS COVID-19 assay using a standard curve generated by spiking a known amount of heat-inactivated virus into healthy donor saliva and performing serial dilutions in healthy donor saliva. Results from this analysis demonstrated good linearity (R2 = 0.90) across a broad range of virus concentrations (1-107 copies/μL; Fig. 2A), with a LOD (24) of 0.05 copies/μL. Subsequent CRISPR-FDS analysis of 20 replicate samples containing 0.05, 0.1 and 0.25 copies/μL detected positive signal in all samples, suggesting the actual LOD is < 0.05 copies/μL (Fig. 2B). CRISPR-FDS demonstrated complete concordance with RT-qPCR when analyzing saliva aliquots spiked with or without virus, at concentrations matching 1× to 2× the LOD (0.05 and 0.1 copies/μL, respectively) of the CRISP-FDS assay (Fig. 2C). Positive and negative percent agreement (PPA and NPA) estimates remained high after adjusting for sample size (100%; 95% CI: 85.8 – 100). Comparison of CRISPR-FDS results for paired saliva and nasal swab samples obtained from a cohort of 31 individuals screened for COVID-19 (Fig. 2D) indicated that viral loads were similar in these samples and demonstrated reasonable correlation (r= 0.8029, p<0.0001), and SARS-CoV-2 RNA levels remained stable in saliva stored at 4°C for up to 7 days after collection (fig. S2). Fig. 2 Analytical and clinical validation of the saliva-based CRISPR-FDS assay. (A). CRISPR-FDS signal from aliquots of healthy donor saliva spiked with a broad range (1 – 107 copies/μL) of SARS-Cov2 virus. (B). Reproducibility of CRISPR-FDS positive results in saliva containing low viral loads. (C) Correspondence and positive and negative percent agreement (PPA and NPA) of RT-qPCR and CRISPR-FDS results for aliquots of saliva from a healthy individual spiked with or without SARS-CoV-2 virus. (D) CRISPR-FDS correlation of paired saliva and nasal swab samples from 31 COVID-19 cases, indicating the linear regression line (solid) and the limits of its 95% confidence intervals (dashed). Data represent the mean±SD of three replicates. Saliva virus levels are elevated throughout infection in a rhesus macaque model of COVID-19 Saliva from COVID-19 patients contains SARS-CoV-2 RNA (25–27), and saliva and nasal samples exhibit high PPA when analyzed within 7 days of COVID-19 symptoms onset, which declines thereafter, with an indication that saliva samples may remain positive longer (28). Given the uncertainty involved in human exposure events, it is not possible to evaluate the relative diagnostic utility of saliva and nasopharyngeal swab samples early in infection. To address this question, we analyzed paired nasal and oropharyngeal (saliva analog) swabs obtained from seven non-human primates before and after SARS-CoV-2 infection. CRISPR-FDS determined that mean SARS-CoV-2 RNA levels were 3.6-fold to 124-fold higher, and more stable, in oropharyngeal versus nasal swab samples at all times points after infection (Fig. 3A). This data, and limited human data (28), suggest that saliva may represent a more robust diagnostic sample than nasal swabs both early and later in infection. Fig. 3 CRISPR-FDS detection of SARS-Cov2 RNA in animal and patient samples. (A) CRISPR-FDS results for RNA isolated from nasal and oropharyngeal (saliva surrogate) swab samples collected from a non-human primate model of COVID-19 at baseline (14 days before SARS-CoV-2 infection) and at 7, 14, 21 and 28 days post-infection. Data represent the mean ± SD of 7 animals. (B) Chest computed tomography (CT) scan and (C) Saliva CRISPR-FDS results for a patient who demonstrated pathology consistent with COVID-19 but had two negative nasal swab RT-qPCR results, compared to results from saliva of a healthy donor (HD) with a negative nasal swab RT-qPCR result. and negative control (NC, no template added) and positive control samples (102 copies synthetic SARS-CoV-2 RNA). (D) CRISPR-FDS analysis of SARS-CoV-2 viral load distributions in paired saliva and nasal swabs samples from 103 individuals undergoing screening for COVID-19. (E) CRISPR-FDS correlation of paired saliva and nasal swab samples from 103 COVID-19 cases. Data represent the mean±SD of three replicates. Anecdotal evidence also suggests that saliva CRISPR-FDS results may have greater diagnostic sensitivity than nasopharyngeal swab RT-qPCR results, as illustrated by a COVID-19 patient whose nasal and nasopharyngeal swabs tested negative by RT-PCR, but whose saliva tested positive by CRISPR-FDS (29). In this case, a 53-year old woman with Philadelphia-negative B cell acute lymphoblastic leukemia presented with fever, shortness, of breath, cough, and tachycardia. A chest X-ray revealed bilateral lung infiltrates that were confirmed by a chest computed tomography scan which revealed ground glass opacities suggestive of COVID-19 (Fig. 3B). However, nasal and nasopharyngeal RT-PCR swab tests (Abbott and Roche) that were conducted as her respiratory status deteriorated to require 4L/min oxygen via nasal cannula were negative for SARS-CoV-2. Additional infectious causes were ruled out using the BioFire respiratory panel, serum fungal markers, and serum tests for cytomegalovirus, Epstein-Barr virus, adenovirus, Legionella, and Cryptococcus. Continued suspicion of COVID-19 lead to investigational RT-PCR CRISPR-FDS testing of her saliva, which was determined to be SARS-CoV-2 positive (Fig. 3C). This patient was transferred to the COVID-19 ward for isolation where she received one unit (200 mL) of COVID-19 convalescent plasma. Within 24 hours, she reported improvements in shortness of breath and cough, exhibited fever resolution, and reduction in her oxygen requirements leading to her eventual discharge without supplemental oxygen. This report, albeit anecdotal, supports the ability of a saliva CRISPR-FDS assay to detect COVID-19 cases missed by RT-PCR assay. In this case, the CRISPR-FDS saliva assay results were instrumental in identifying the false negative RT-PCR results to allow rapid initiation of SARS-CoV-2 directed therapy with convalescent plasma to reverse COVID-19 pathology. Subsequent CRISPR-FDS analysis of 103 paired saliva and nasal swab samples obtained from individuals screened for COVID-19 detected SARS-CoV-2 RNA in more saliva than nasal swab samples (44 versus 28, Fig. 3D). Viral load was strongly correlated in the 24 individuals with two positive samples (Fig. 3E) . Design and diagnostic performance of the COVID-19 CRISP-FDS saliva assay read on a chip These results support the hypothesis that saliva is a valid specimen for COVID-19 diagnosis and can yield results comparable or superior to those obtained with nasopharyngeal or nasal swab samples to reduce the materials, expertise and infrastructure required to for sample collection. However, this assay still requires a benchtop fluorescence reader for assay evaluation and quantification, limiting its utility to sites equipped with such devices. Smartphone-based devices have become an appealing handheld platform to read multiple different assay types (30–34), and we hypothesized that a smartphone device could similarly allow sensitive and quantitative readout of saliva CRISPR-FDS assays at sites lacking basic laboratory equipment or infrastructure. Such an approach would require that the CRISPR-FDS assay be reformatted to be easily read by a smartphone camera. To address this issue, we designed and fabricated a proof-of-concept compact assay chip (25 × 35 × 4 mm) suitable for an on-chip CRISPR-FDS saliva assay inserted into a smartphone device functioning as a fluorescence microscope for capture by the field of view of its camera. This prototype chip consisted of a layer of polydimethylsiloxane (PDMS) mounted on a glass microscope slide. PDMS was selected for this application because it is a chemically inert and optically clear silicone elastomer that can spontaneously adhere to glass surfaces after plasmonic oxidation, because it allows assays wells to be excited by low angle laser illumination, and because this inexpensive PDMS/glass format (∼$0.7/chip) can be readily modified to improve assay functionality. Our chip design contained five reaction wells (I.D. = 3.5 mm, maximum volume ≈ 28 μL) to allow the analysis of five assays in parallel (e.g., three test wells, one PC well and one NC well), where wells were designed to contain sufficient volume for sensitive detection. Reaction wells were arranged in a pentagonal array illuminated by a laser diffused to cover the ~20 × 20 mm field of view of the smartphone camera (Fig. 4A-C). A pentagonal array was chosen to minimize illumination differences in our proof-of-concept experiments, more compact arrays containing more wells could be employed to simultaneously analyze samples from multiple individuals and/or to accommodate a standard curve to quantify viral load. Alternate designs could also employ microfluidic channels to load multiple wells from a single inlet port and employ film to seal the chip after sample loading to prevent environmental contamination by assay amplicons. To verify the utility of this chip design, we employed an on-chip CRISPR-FDS assay to analyze saliva from 12 COVID-19 patients and 6 healthy controls. Images from this proof-of-concept assay approach that were captured and analyzed by a fluorescent microplate reader (Fig. 4D-E) distinguished saliva from patients with positive and negative nasal RT-qPCR results, supporting the feasibility of this on-chip approach for COVID-19 diagnosis. Fig. 4 CRISPR-FDS chip prototype design and on-chip assay results. (A) Schematic of the on-chip design, (B) a prototype chip example, and (C) the assay readout schematic. (D-E) Fluorescent on-chip CRISPR-FDS assay images and signal for saliva from 11 patients diagnosed with COVID-19 and 7 patients diagnosed as non-COVID-19 cases by their nasal RT-qPCR results. (D) Fluorescent images were scanned and merged by a Cytation 5 Cell Imaging Multi-Mode Reader with 525nm filter and (E) assay fluorescence intensity was analyzed by NIH Image J image analysis software. Data represent the mean ± SD of 3 replicates. Photo Credit: Bo Ning, Tulane University. Analytical and diagnostic performance of on-chip assays read by a smartphone device We next developed a smartphone fluorescence microscope device to read the on-chip CRISPR-FDS assay. This device incorporated a smartphone socket, external lens filter, laser diode powered by AAA batteries, a power switch, chip slot, and an emission filter for the smartphone camera (Fig. 5A). This integrated system was designed to utilize a straightforward workflow, in which a typical saliva volume of 0.5~3 mL (35) is collected in a tube prefilled with 3 mL of lysis buffer, which is then capped and heated at >37°C for ≥5 min, after which ~5 μL of the lysed sample is added to each sample well of an assay chip containing 10 μL/well of pre-mixed RPA and CRISPR solution. This chip is then incubated for ≥10 min at room temperature and then inserted into the smartphone reader, the laser diode is turned on, and assay chip images are captured by the smartphone camera (Fig. 5B). Fig. 5 Design of a smartphone-based assay reader and its analytical and diagnostic performance when employed to read on-chip CRISPR assays. (A) Schematic of a 3D-printed smartphone fluorescence reader. (B) Workflow of a saliva-based on-chip CRISPR-FDS smartphone assay. (C) An example CRISPR-FDS assay fluorescent signal image captured images captured with a 525nm filter with cellphone. (D) Standard curve of the on-chip CRISPR-FDS saliva test read by the smartphone device. (E) Comparison SARS-CoV-2 viral load in saliva samples read by the smartphone device and by RT-PCR. Fluorescence intensity in smartphone images was analyzed by NIH Image J image analysis software. (F) Correlation of smartphone CRISPR-FDS and RT-qPCR assay results for saliva samples from 103 COVID-19 cases, indicating the linear regression line (solid) and the limits of its 95% confidence intervals (dashed). Data represent the mean±SD of three replicates. Photo Credit: Bo Ning, Tulane University. The field of view (FOV) of this device was increased relative to previous smartphone microscope designs (36) by adding an external lens with a 50 mm focal length. This yielded a FOV compatible with diameter of the reaction well array on our assay chip without producing significant aberration. This device also employed a 100 mW laser diode with a high incidence angle to allow sensitive detection of reaction products while minimizing background noise (Figs. 5C and S3). We examined the analytical performance of this device by analyzing on-chip assays of a SARS-CoV-2 RNA concentration curve generated serially diluting heat-inactivated SARS-CoV-2 virus in healthy donor saliva. This standard curve demonstrated good linearity (R2 = 0.91) over a broad viral concentration range (1 – 105 copies/μL) and a calculated LOD of 0.38 copy/μL when read on the smartphone device, (Fig. 5D) (24). We then employed both CRISPR-FDS and RT-qPCR to blindly analyze 103 saliva samples from individuals screened for COVID-19 (Fig. 5E), and found that the CRISPR-FDS plate reader and smartphone assays and the standard RT-qPCR assay detected similar numbers of SARS-CoV-2 positive saliva samples (43 versus 44) (Table S1). In an analysis using RT-qPCR as the reference standard (Table S1), CRISPR smartphone results exhibited a 1.3% false positive rate with saliva but complete concordance with RT-qPCR results for swab samples, while CRISPR plate reader results perfectly matched RT-qPCR saliva results, but exhibited a 2.3% false negative rate with nasal swab samples. Viral load was strongly correlated in the 43 saliva samples that tested positive by both the on-chip smartphone assay and conventional RT-PCR analysis (Fig. 5F), and exhibited similar mean values (3803 versus 1797 copies/μL).

Discussion New COVID-19 tests should ideally address several unmet needs. Rapid and ultrasensitive assays are needed to increase testing capacity and ideally should not require significant equipment, technical expertise, or utilize diagnostic samples that must be collected by medical professionals. Sensitive and quantitative testing capacity is also needed to evaluate viral endpoint measures in a multitude of ongoing or planned clinical and animal studies for CoVID-19-directed therapeutics or vaccines. Recent studies have suggested that saliva may represent an ideal alternative diagnostic specimen for such assays. SARS-CoV-2 RNA was successfully detected in saliva samples collected from the deep throat region, oral cavity and salivary gland of COVID-19 patients, (25–27) with viral load reported to be highest early after symptoms onset (37). Moreover, saliva and nasopharyngeal samples are reported to exhibit very high positive percent agreement (PPA; 96.6%) when samples were collected within 7 days of symptoms onset, after which saliva appears to remain positive longer (28). Virus loads during early infection cannot be accurately determined in COVID-19 patients at specific time points post-infection due uncertainty regarding initial infection events. However, our non-human primate data indicate that the SARS-CoV-2 viral load was significantly higher in oropharyngeal versus nasal swab samples at all time points when these samples were analyzed by CRISPR-FDS assay. SARS-CoV-2 RNA levels in analogous samples from a rhesus macaques infected with a high intratracheal dose (106 × TCID 50 ) peaked at 3 days post-infection and declined to undetectable levels within 10 days post-infection (38); however, these animals developed mild to moderate disease and thus do not mimic the animals in our study, which developed asymptomatic infections. . We also observed that saliva samples were more frequently SARS-CoV-2 positive than nasal swab samples when paired samples were tested by CRISPR-FDS, and that saliva samples had higher mean viral loads when both samples were positive. Taken together, these results suggest that saliva may be a more sensitive diagnostic sample than nasal swabs. Saliva levels of SARS-CoV-2 may also represent an important surrogate of virus infectivity, as viable virus can be easily cultured from saliva and saliva droplets are an important mechanism for virus transmission (25, 39). Monitoring viral shedding in saliva should thus be of significant interest as a potentially more direct and accurate means to evaluate infectivity in clinical practice and research studies. Our results demonstrate that our saliva-based on-chip CRISPR-FDS assay for COVID-19 exhibits complete concordance with RT-qPCR when analyzing saliva samples spiked with SARS-CoV-2 concentrations that fall within the linear range of the RT-PCR assay, an estimated 0.38 copies/μL LOD, and a broad linear range (1-105 copies/μL). Notably, this on-chip assay does not require RNA isolation but exhibits a LOD similar to RT-qPCR (0.38 copies/μL versus 1 copy/μL) and greater than CRISPR-based COVID-19 assays proposed for point-of-care diagnosis (4~10 copies/μL), all of which require separate RNA isolation procedures (14, 21–23). This assay platform has several features that should render it suitable for use in a variety of point-of-care testing environments, since it: analyzes saliva samples that can be collected by the subject being tested to reduce the demands upon medical personnel; exhibits robust performance in response to large variations in sample dilution and denaturation and CRISPR-FDS reaction temperatures and times; and utilizes an inexpensive and highly portable smartphone-based reader, which could also speed and simplify coded data reporting from remote testing sites. Notably, the estimated sensitivity of our prototype smartphone device for SARS-CoV-2 in on-chip assays approaches the sensitivity we detect when in off-chip assays are read by a fluorescent microplate reader (0.38 versus 0.05 copies/μL), supporting the potential for broad use of this platform in screening and diagnosis. This sensitivity was achieved by low incident angle illumination of the assay chip by a 100 mW laser diode powered by AAA batteries that achieved high excitation intensity and signal-to-noise conditions for assay well image capture. Sample focusing and image acquisition were achieved by the built-in smartphone camera app, eliminating the need for the mechanical focusing, and thus reducing weight and cost while enhancing the optical stability and user-friendliness of the device. This proof-of-concept assay platform demonstrates the basic functionality required for a rapid ultrasensitive assay suitable for use in resource-limited point-of-care settings or screening sites. However, there are several refinements that could be made to further improve the user-friendliness and resource requirements of the current device. For example, our current protocol requires that saliva be lysed and then added to the chip for analysis, as do RT-RPA CRISPR-FDS reaction mixtures and controls, contributing additional sources of potential variation and error. We are therefore investigating the potential to control sample lysis and subsequent RT-PCR and CRISPR-FDS reactions on a microfluidic chip. In our anticipated next generation chip and device design, saliva would be directly applied to a sample loading site on an integrated chip that would contains pre-loaded reagents and sample controls. Microfluidic channels on this chip would regulate the flow and mixing of reaction samples, while heating elements controlled by the smartphone would precisely regulate reaction temperatures. These chips could also be barcoded to facilitate data reporting. Finally, a custom smartphone app would regulate chip temperature zones for the lysis, RT-RPA, and CRISPR-FDS reactions, automatically capture assay well images using the smartphone camera and analyze this data, and permit secure wireless reporting of assay data from remote sites to central locations to support telehealth efforts and provide aggregate data to governmental organizations tasked with making public health decisions. We believe this smartphone platform, a similar future application, offers the potential to rapidly expand COVID-19 screening capacity, and potentially simplify the verification of contact tracing, to improve local containment and inform regional disease control efforts.

Materials and Methods Key Materials The SuperScript IV One-Step RT-PCR System (1235820) and nuclease-free water (4387936) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA). The EnGen® Lba Cas12a (M0653T) and NEBuffer 2.1 (B7202S) were purchased from New England Biolabs (Ipswich, USA). Primers, gRNA, probes (Table S2), used in the study were synthesized by Integrated DNA Technologies, Inc. (Coralville, IA). PDMS elastomer (Sylgard 184) was purchased from Dow Corning (Midland, MI), microscope glass slides were obtained from Fisher Scientific (Hampton, NH), and plasmonic oxidation was performed with a benchtop plasma cleaner PDC-001 purchased from Harrick plasma (Ithaca, NY). Nucleic Acid Extraction For assays analyzing isolated saliva RNA, total RNA was isolated from 100 μL of each saliva sample using a Quick DNA/RNA viral kit (Zymo; Hilden, Germany) and stored at -80°C until analysis. RT-qPCR RT-qPCR was performed with the CDC 2019-Novel Coronavirus (2019-nCoV) Real Time RT-qPCR Diagnosis Panel. In each reaction, 5 μL of RNA sample was mixed with 1 μL of Combined Primer/Probe Mix, 5 μL of TaqPathTM 4X 1-Step RT-qPCR Master Mix, and 9 μL of nuclease-free water. RT-qPCR reactions were performed using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific Inc., Waltham, USA) using the reaction conditions specified for this assay. RT-RPA amplification Recombinase polymerase amplification (RPA) pellets from the TwistAmp® Basic kit (ABAS03KIT; TwistDx Limited; Maidenhead, UK) were resuspended in 29.5 μL of the supplied Rehydration Buffer, and 11.8 μL of this RPA solution, 0.5 μL of forward primer (10 μM), 0.5 μL of reverse primer (10 μM), 3.2 μL of nuclease-free water, 4 μL of magnesium acetate (MgOAc; 280 mM), 1 μL SuperScript IV reverse transcriptase and 5 μL of 60 μL isolated RNA sample were mixed and incubated at 42°C for 20 min. CRISPR-FDS reactions For microplate-based CRISPR-FDS assays, 20 μL of a completed RT-RPA reaction was transferred to a 96-well half-area plate and mixed with 10 μL of a CRISPR reaction mixture containing 3 μL of 10× NEBuffer 2.1, 3 μL of gRNA (300 nM), 1 μL of EnGen® Lba Cas12a (1 μM), 1.5 μL of fluorescent probe (10 μM), and 1.5 μL of nuclease-free water. After incubation at 37°C for 20 min in the dark, fluorescence signal was detected using SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, LLC., San Jose, USA). For the Cas12a substrate-dependent kinetics study, the CRISPR-FDA reaction contained 1:15, 1:20, and 1:25 molar ratios of Cas12a/gRNA to fluorescent probe. For the temperature-dependent kinetics study, reactions were performed using a 1:25 Cas12a/gRNA to fluorescent probe ratio at 22°C, 37°C, and 42°C. RNA extraction-free saliva assay QuickExtract DNA Extraction Solution (Lucigen) was mixed with saliva samples as indicated to release viral RNA, since this solution is compatible with PCR, RPA and CRISPR reactions (40). Saliva and lysis buffer mixtures were then incubated at the temperatures and times specified, after which 5 μL of the lysed sample was mixed with a RT-RPA solution described above and incubated at 37°C to amplify a SARS-CoV-2 ORF1ab gene target, which was detected by addition of a 10 μL mixture containing a CRISPR-FDS reactions mixture described above. For the on-chip CRISPR-FDS assays, 10 μL of an RT-RPA CRISPR solution generated by mixing equal volumes of the RT-RPA and CRISPR-FDS reaction mixtures was pre-loaded into each chip well, after which 5 μL of saliva lysate or purified RNA was loaded into these wells, and the chip was incubated at ≥22°C for ≥5 min. Standard curve, LOD, and LOD detection rates Standard curve were generated by spiking a known amount of heat-inactivated virus into healthy donor saliva and then serially diluting this starting sample with healthy human saliva, as indicated for the respective assay. LOD was calculated using the mean and SD of the zero control (blank) and lowest concentration standard following the formula: LOD = mean blank + 1.645 (SDblank) + 1.645 (SD low concentration sample) (24). To assess detection rates near the estimated LOD, healthy donor saliva was spiked with heat-inactivated SARS-CoV-2 virus at 0.25 copy/μl, and serially diluted to 0.1 and 0.05 copy/μl concentrations. RNA was extracted from each of these dilution samples by three different individuals to generate three RNA batches. For RT-RPA CRISPR-FDS assays, each extracted RNA batch was analyzed in 20 replicates for a total of 60 replicates among the three batches generated at each concentration. A positive sample was defined as any sample with a CRISPR-FDS signal that was greater than the mean signal of the negative control plus three times its SD. Cross-reactivity (Analytical Specificity) In silico testing. A total of 39 bacterial/viral/fungal strains have been analyzed in silico. NCBI BLAST tool was used to check for cross-reactivity of the primer/gRNA sets of the SARS-CoV-2 assay against the non-redundant nucleotide database. The default parameters of BLAST tool were used except for the “organism.” The search was limited to using the taxonomy ID (taxid/txid) of the respective pathogen. Each primer and gRNA were compared against all the available genomic sequences of a certain taxid. Wet testing. To confirm the in silico data, we conducted the wet testing of the high risk pathogenic microorganisms commonly seen in the respiratory tract. Each microorganism listed in Table S3 was analyzed in triplicate with our SARS-CoV-2 CRISPR by spiking diluted organism stock into lysis treated pooled nasopharyngeal swab matrix. Non-human Primate Model Ethics Statement. The Institutional Animal Care and Use Committee of Tulane University reviewed and approved all the procedures for this experiment. The Tulane National Primate Research Center is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC). All animals are cared for in accordance with the NIH guide to Laboratory Animal Care. The Tulane Institutional Biosafety Committee approved the procedures for sample handling, inactivation, and removal from BSL3 containment. Virus Information. SARS-CoV-2 isolate USA-WA1/2020 was acquired from BEI Resources, and the harvested stock was determined to have a concentration of 1 × 106 TCID 50 /ml. The virus was passaged in VeroE6 cells in DMEM media with 2% FBS sequence confirmed by PCR and/or Sanger sequencing. Plaque assays were performed in Vero E6 cells. Animals and Procedures. A total of seven non-human primates aged >11 years (2 male and 1 female African green monkey and 2 male and 2 female Indian rhesus macaques) were analyzed in this study. Animals were exposed to SARS-CoV-2 either by multi-route combination or small particle aerosolization. Animals exposed by aerosol received an inhaled dose of ~2.5x104 PFU. Animals exposed by multi-route combination (oral 1mL; nasal 1mL; intratracheal 1mL; conjunctiva 50uL per eye) received a cumulative dose of 3.61x106 PFU. Animals were observed for 28days post infection including twice daily monitoring by veterinary staff. Design of the smartphone device for quantification of the on-chip CFRISPR-FDS assay. This device is comprised of two components: a Samsung Galaxy S9 smartphone with a 12.2 megapixel (5312 × 2988) 7.06mm CMOS image sensor and a rear camera with a 26 mm focal length; and a custom printed fluorescent microscope interface that was designed in Autodesk Inventor, prepared by 3D printing (StrataSys uPrint SE plus) and then fitted with optomechanical attachments. A blue laser diode (465nm, >100mW, DTR’s Laser Shop) powered by 3 AAA batteries was connected to a heatsink and mounted to the 3D-printed base, as was a convex lens (f 2 = 50mm, Edmund Optics, #38-296) for signal collection with a demagnification index calculated as M = f 1 /f 2 ≈ 0.52, and a 525 nm band-pass filter (Edmund Optics, #86-354) that was placed in front of the smartphone camera as an emission. This device was also fitted with a sample tray to accurately position the assay chip during its readout. Capture and analysis of smartphone CRISPR-FDS reaction well images. Assay chips containing completed CRISPR-FDS reactions were inserted into the smartphone reader, and images of chip fluorescent signal were captured using the Samsung Galaxy S9 smartphone camera app Version 9.0.05, using the manual focusing function in this app. All samples analyzed on the Samsung smartphone device were imaged using 1/15s integration time and an ISO value of 100, and captured images were stored using a lossless raw format (DNG file). Raw smartphone DNG images were first transferred to computer through a USB cable and converted to RGB TIFF images via DC RAW V 1.5.0. A monochromic TIFF image was generated by extracting the green (G) channel values for further analysis, where pixel intensity from this monochromic image was then measured and analyzed using ImageJ. Clinical samples. Nasal swab and saliva specimens were collected at Tulane Medical Center in New Orleans, LA from March 30 to July 16, 2020 with written informed consent, in accordance with an approved IRB protocol, and the COVID-19 status of the donors was determined based on clinical indications and current CDC guidance, and all nasal swab samples were tested with the CDC 2019-nCoV real-time RT-PCR Diagnostic Panel (EUA). Saliva from of a Philadelphia-negative B cell acute lymphoblastic leukemia patient analyzed in this study, and previously reported in a case study, (29) was provided by Dr. Nakhle Saba under a separate IRB. Clinical specimens from COVID-19 infected subjects were processed in an enhanced BL2/BL3 space at Tulane University in accordance with a protocol approved by the Institutional Biosafety Committee. Statistical analysis. CRISPR-FDS assay signal was expressed as the mean of ≥ 3 independent reactions ± SD. GraphPad Prism 8 was used to calculate one-way ANOVA determine the optimized condition of RT-RPA and calculate linear regression of standard curve. Multiple group comparisons were conducted using one-way ANOVA. Differences were considered statistically significant at P < 0.05.

Supplementary Materials Supplementary material for this article is available at: https://advances.sciencemag.org/cgi/content/full/sciadv.abe3703/DC1

https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Acknowledgments: :Funding: This study was supported by R01AI113725, R01AI122932, and R21Al126361 from National Institute of Allergy and Infectious Diseases, R01HD090927 from a National Institute of Child Health and Human Development and W8IXWH1910926 from Department of Defense. T.Y.H. also wants to thank the generous support of Weatherhead Presidential Endowment fund. T.Y., S.Z., and Q.W. also would like to thank the support from the National Science Foundation (Award #1944167). Author contributions: B.N. and T.Y.H conceived the study, generated the hypotheses and designed the experiments. B.N., T.Y, S.Z. and Z.H. performed all the experiments and analyzed the data. B.N. and C.J.L wrote the manuscript. D.T, Z.L, A.N., X.M.Y, and N.S.S conceived and designed the clinical study; N.G, K.H, B.T, C.J.R, and J. R conceived and designed the non-human primate study; B.N, Q.W. C.J.L and T.Y.H performed analyzed and interpreted the clinical data; all authors contributed to manuscript review, revision, and finalization. Competing interests: Tony Ye HU, Bo NING and Zhen HUANG are inventors on a provisional patent application related to this work filed by Tulane University (NO. 63/027,530, Filed on May 20, 2020). The authors declare no other competing interests.Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials.

iPuntMidgets on January 3rd, 2021 at 03:54 UTC »

A lot of these technologies will be useful for future pandemics which, unfortunately, will probably be a reality.

NavyCMan on January 3rd, 2021 at 02:20 UTC »

Can someone help the slower kids in class? Like me?

mvea on January 3rd, 2021 at 01:37 UTC »

I’ve linked to the open access full-text source journal article in the post. The citation of the article is here:

A smartphone-read ultrasensitive and quantitative saliva test for COVID-19

Science Advances 11 Dec 2020: eabe3703

DOI: 10.1126/sciadv.abe3703

Abstract

Improved point-of-care COVID-19 assays are urgently needed to improve disease control efforts, but current assays lack the sensitivity of the RT-PCR gold-standard. Herein we describe the development of a portable, ultrasensitive saliva-based COVID-19 assay with a 15 minute sample-to-answer time that does not require RNA isolation or laboratory equipment. This assay uses CRISPR/Cas12a activity to enhance signal from an amplified viral RNA target, which is stimulated by laser diode integrated into a smartphone-based fluorescence microscope readout device. This device robustly quantified viral load over a broad linear range (1-105 copies/μL) and exhibited a limit of detection (0.38 copies/μL) below that of the RT-PCR reference assay. SARS-CoV-2 RNA levels read by CRISPR were similar in patient saliva versus nasal swab samples and viral loads measured by RT-PCR and the smartphone-read CRISPR assay demonstrated good correlation, supporting the potential utility of this portable assay device for saliva-based point-of-care COVID-19 diagnosis.